Table 2 Characteristics of the gene-specific qPCR assays
Gene name (synonym) | Access n° Gene ID | Gene location | Primer sequence 5'-3' | Amplicon size Melting T°c | PCR efficiency |
|---|---|---|---|---|---|
KRT4 | NM_002272 | 12q12-q13 | f: tcaacaacaagtttgcctc | 185 | 1.94 |
keratin 4 | 3851 | r: gtcattgcccaaggtatcta | 90 | ||
IL1RN | NM_173841 | 2q14.2 | f: cctgtcctgtgtcaagtctg | 257 | 1.80 |
interleukin 1 receptor antagonist | 3557 | r: cgtcctcctggaagtagaat | 90 | ||
KRT13 | NM_153490 | 17q12-q21.2 | f: tctctgtcttgctggtctga | 234 | 1.88 |
keratin 13 | 3860 | r: atgaagaggagatgaaggaa | 89 | ||
MMP1 | NM_002421 | 11q22.3 | f: aaagacagattctacatgcg | 237 | 1.92 |
matrix metallopeptidase 1 | 4312 | r: tgcttcacagttctaggga | 85 | ||
PLAU | NM_002658 | 10q24 | f: ggactacatcgtctacctgg | 230 | 1.90 |
plasminogen activator, urokinase | 5328 | r: caaactggggatcgttatac | 88 | ||
SPARC | NM_003118 | 5q31.3-q32 | f: ggtgactgaggtatctgtgg | 245 | 1.85 |
secreted protein acidic cysteie-rich | 6678 | r: aggtcttgttgtcattgctg | 90 | ||
TGM3 | NM_003245 | 20q11.2 | f: cactctccaatggcagtagt | 215 | 1.93 |
transglutaminase 3 | 7053 | r: cataaagacgctatccacat | 88 | ||
FN1 | NM_212482 | 2q34 | f: tgacacttatgagcgtcct | 234 | 1.81 |
fibronectin 1 | 2335 | r: aaacacttctcagctatggg | 86 | ||
MAL | NM_002371 | 2cen-q13 | f: ataaagccgcagtagaactt | 181 | 1.95 |
mal, T-cell differentiation protein | 4118 | r: agagtaaacacagcacccac | 84 | ||
ACTB | NM_001101 | 7p15-p12 | f: tggctggggtgttgaaggtct | 238 | 1,89 |
actin, beta | 60 | r: agcacggcatcgtcaccaact | 90 | ||
B2M | NM_004048 | 15q21-q22.2 | f: cagcgtactccaaagattca | 240 | 1.99 |
beta-2-microglobulin | 567 | r: gaatgctccactttttcaat | 90 | ||
RPS18 | NM_022551 | 6p21.3 | f: agcttgttgtccagaccatt | 187 | 1.84 |
ribosomal protein S18 | 6222 | r: tgaggaaagcagacattgac | 87 |