Figure 7

FER, CTNND1, CAV1 and their phosphorylated version expression in two HCC cell lines. First, equal protein lysates from two HCC cells lines were subjected to SDS-PAGE electrophoresis and subsequent Western blotting using anti-FER, CTNND1, CAV1 and actin antibodies (lower panel). Then in parallel experiment, equal protein lysates from two cell lines were immunoprecipitated using anti-pTyr 4G10 and pTyr100 antibodies, followed by reacting with FER, CTNND1 and CAV1 antibodies in Western blot assay (upper panel). This represents three independent experiments.