Figure 3

Activation of NF-kappa B pathway in GA-induced apoptosis. a: Nuclear translocation of p65 subunit of NF-kappa B in Tca8113 cells after treatment with GA or TNF-α (A: control, without nuclear staining, B: control, with nuclear staining, C: 12 hours treatment with 4 μM GA, without nuclear staining, D: 12 hours treatment with 4 μM GA, with nuclear staining, E: 60 minutes treatment with 50 ng/ml TNF-α, without nuclear staining, F: 60 minutes treatment with 50 ng/ml TNF-α, with nuclear staining). b: EMSA analysis of NF-kappa B binding activity in Tca8113 cells after 4 to 16 hours of treatment with 4 μM GA.