Figure 2

Apoptotic changes in GA-treated OSCC cells. a: Morphological changes of Tca8113 cells under transmission electronic microscopy (A: control, B: early-stage apoptosis, C: middle-stage apoptosis, D: late-stage apoptosis, 4000 × 10 original magnification). b: Flow cytometry analysis of DNA content in Tca8113 cells treated with 4 μM GA for 24 hours to 72 hours (*: the sub-G1 peak). Percentages of sub-G1 peak are labeled. c: The rates of cell apoptosis were measured by annexin V and PI staining and detected by flow cytometry. Percentages of early apoptosis (Lower Right region) and late apoptosis (Upper Right region) are labeled. Images are representative of three independent experiments.