Figure 9
The time course of estrogenic phospho-activation of pElk-1. Time-dependent activation of Elk-1 by phosphorylation was measured by a quantitative plate immunoassay for phospho-Elk-1 (pELK-1) in cells treated with (A) 10-7 M cholesterol (negative control), (B) 20 nM TPA (positive control), (C) 10-9 M E2, (D) 10-8 M coumesterol, (E) 10-7 M daidzein, (F) 10-7 M genistein, (G) 10-7 M trans-resveratrol. * = p < 0.05 compared to vehicle only treated cells.