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Table 3 Details of protocols used for fluorescent co-localisation.

From: Expression of oestrogen receptors, ERα, ERβ, and ERβ variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERα

ERβ1+PR

ERα +PR

COX2+ ERα

Citrate retrieve

Citrate retrieve

Citrate retrieve

Methanol/peroxide block

NGS block

NRS block

NGS block

Avidin block

Avidin block

Avidin block

Biotin block

Biotin block

Biotin block

ERα 1:20

COX-2 1:60

PR 1:50

GAM 488

RAGB

GARB

NGS block

Streptavidin 546

Streptavidin 546

PR 1:50

NGS

NGS block

GARB

ERα 1:20

ERβ1 1:200

Streptavidin 546

GAM 488

GAMP

To-Pro

To-Pro

Tyramide fluoresein

  

To-Pro

  
  1. The protocol for each combination of stains is given in the vertical columns; all sections are pre-blocked with normal goat serum