Figure 4

(A&B): Band supershift assay showing composition of AP-1 complexes in HPV positive and HPV negative esophageal cancer. 10 μg of nuclear protein extracts from HPV16-positive (A) or HPV-negative (B) ESCC biopsies were co-incubated with γ³²P-labeled double stranded AP-1 probe. Oligonucleotides and specific antibodies (2 μg each) to all AP-1 family proteins as indicated and analyzed on a 4.5% PAGE. The intensities of the shifted and super-shited bands were quantified. Supershift was calculated as a percent loss of band intensity indicating AP-1 binding in reaction having no antibody.