Figure 4

Impact of leukocyte age and recipient age on donor leukocyte functionality. A) Survival curves. The ages of "young" C57BL/6 SR/CR donors were 4.8 ± 0.5 months and "old" donors were 22.9 ± 3.5 months. The ages of "young" C57BL/6 WT recipients were 2 months and "old" recipient mice were 24 months. Survival percentages of recipient mice are shown: young to young (Orange triangle, n = 16), young to old (Green square, n = 15), old to old (Red circle, n = 16) and old to young (Blue asterisk, n = 16). The adoptive transfer recipients were then challenged 1 day after the adoptive transfer, then approximately every 30 days thereafter for the survivors. The area to the left of the dashed line represents the impact that the age of the donor leukocytes has on the survival of the recipient. B) Bar chart. The same experimental conditions as described in figure 4A after each S180 challenge. The survival for each experimental group after the subsequent challenges was as follows: First S180 challenge: 88% for young to young, 73% for young to old, 25% for old to young, and 25% for old to old; Second S180 challenge: 69% for young to young, 50% for young to old, 25% for old to young, and 25% for old to old; Third S180 challenge: 69% for young to young, 33% for young to old, 25% for old to young, and 13% for old to old; Fourth S180 challenge: 69% for young to young, 13% for young to old, 25% for old to young, and 13% for old to old.