Figure 1
Functionality of donor leukocytes in mismatched recipients. The pooled donor leukocytes were evenly given to the recipients intraperitoneally (IP). After transfers of donor leukocytes, recipient mice were challenged with 1 × 106 S180 cells IP. The percent survival of the recipient mice was presented to indicate the functionality of the transferred donor leukocytes after the first challenge (left bars, 1 day after the transfer), second challenge (middle bars, 6 weeks after the transfer) and third challenge (right bars, 12 weeks after the transfer). All recipient mice were WT mice that were sensitive to S180 challenges. For the matched controls, 6 male BALB/c recipients were given male BALB/c SR/CR donor leukocytes and 6 female BALB/c recipients were given female BALB/c SR/CR donor leukocytes. For the sex mismatch, 6 female BALB/c recipients were given male BALB/c SR/CR donor leukocytes; 6 male BALB/c recipients were given female BALB/c SR/CR donor leukocytes. For the MHC mismatch, 6 male C57BL/6 mice were given male BALB/c SR/CR leukocytes and 6 female C57BL/6 mice were given female BALB/c SR/CR leukocytes. For the sex and MHC double mismatch, 6 female C57BL/6 mice were given male BALB/c SR/CR leukocytes and 6 male C57BL/6 mice were given female BALB/c SR/CR leukocytes. The percent survival for each of the adoptive transfers remains unchanged after each subsequent S180 challenge. The overall survival for each experimental group was as follows: matched is 100%, sex mismatch is 83%, MHC mismatch is 58%, and MHC + sex mismatch is 42%.