Table 1 Primer sequences, expected product size in nucleotides (nt) and PCR conditions for each of the studied marker genes.
From: Clinical significance of altered nm23-H1, EGFR, RB and p53 expression in bilharzial bladder cancer
Primer | Sequences | Fragment size | PCR conditions |
|---|---|---|---|
nm23-H1 | 5'-CGCAGTTCAAACCTAAGCAGCAGCTGG-3' 5'-AGATCCAGTTCTGAGCACAGCTCG-3' | 483 bp | 95°C for 5 m followed by: 95°C (30s), 55°C(30s), 72°C(30s) for 32 cycles and 72°C (7 m) |
EGFR | 5'-TGTGAGGTGGTCCTTGGGAATTTGG-3' 5'-TGCTGACTATGTCCCGCCACTGGA-3' | 322 bp | 95°C for 5 min followed by: 94°C (1 m), 66°C (1 m), 72°C (2 m) for 35 cycles and 72°C (7 m) |
β-actin | 5'-ATCTGGCACCACACCTTCTACAATGAGGCTGCG-3' 5'-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3' | 838 bp | 95°C for 5 min followed by: 94°C (45s), 60°C (45s), 72°C (2 m) for 35 cycles and 72°C (7 m) |