Skip to main content

Table 1 Primer sequences, expected product size in nucleotides (nt) and PCR conditions for each of the studied marker genes.

From: Clinical significance of altered nm23-H1, EGFR, RB and p53 expression in bilharzial bladder cancer

Primer Sequences Fragment size PCR conditions
nm23-H1 5'-CGCAGTTCAAACCTAAGCAGCAGCTGG-3'
5'-AGATCCAGTTCTGAGCACAGCTCG-3'
483 bp 95°C for 5 m followed by: 95°C (30s), 55°C(30s), 72°C(30s) for 32 cycles and 72°C (7 m)
EGFR 5'-TGTGAGGTGGTCCTTGGGAATTTGG-3'
5'-TGCTGACTATGTCCCGCCACTGGA-3'
322 bp 95°C for 5 min followed by: 94°C (1 m), 66°C (1 m), 72°C (2 m) for 35 cycles and 72°C (7 m)
β-actin 5'-ATCTGGCACCACACCTTCTACAATGAGGCTGCG-3'
5'-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3'
838 bp 95°C for 5 min followed by: 94°C (45s), 60°C (45s), 72°C (2 m) for 35 cycles and 72°C (7 m)