Effect of expression of Par3 protein on anchorage-independent growth and on three-dimensional growth in Matrigel matrix. (A). Representative images of the wells with colonies developed inside soft agar on day 21. (B). Charts represents the actual number of colonies per well with cut off diameter for counting - 0.2 mm and 0.5 mm. The experiment is representative of three independent analyses with similar results. (C). The effect of PARD3 expression on the three-dimensional growth of LNCaP cells in reconstituted basement membrane (Matrigel). Equal numbers of LNCaP cells expressing Par3 protein or control LNCaP cells transfected with empty vector (EGFP) were seeded over Matrigel pre-coated wells of 24-well plate plates in 2% Matrigel as described in "Methods". The phase contrast images (200×) were taken on every 4 days to track 3D structure formation. The images represent three independent experiments with similar results.