Isolation of CD90
+ stromal cells from cancer tissue. A: Tissue digestion media were probed for TIMP1 and CD90 proteins by Western blot analysis. Sample 00-044NP served as non-cancer control because 08-028NP and 08-032NP were not available. PSA was used as a sample loading control. B: The individual sorted CP stromal transcriptome datasets (second and third columns) contain minimal signals for the epithelial genes that are present in the cancer epithelial cell (CP cancer) transcriptome (first column) in virtual Northern blot format. Affymetrix signal values are represented on a gray scale. C: The expression levels of epithelial genes are below background (≤ 50 RMA) in sorted CP stromal cells.