Summary of Cripto-1 expressions in NPC cell lines and tissues. Expressions of Cripto-1 mRNA and protein in immortalized nasopharyngeal epithelial cell line (NP69), and NPC cell lines (SUNE-1, C666-1, HNE-1, HONE-1, CNE-1 and CNE-2) were examined by real-time RT-PCR (a) and Western blotting (b). Compared to NP69, Cripto-1 expressions were up-regulated in all NPC cell lines (p < 0.05, respectively). (c) 1.5% agarose electrophoresis of Cripto-1 RT-PCR products in NPC tissues. Representative results from 12 NPC cases are shown. β-actin was used as an internal quantitative control. M: Marker; 1~6, 8, 9, 12: positive expression of Cripto-1 mRNA; 7, 10, 11: negative expression of Cripto-1 mRNA.