Figure 3

ICI182780 treatment of MCF-7 cells leads to ERα down-regulation and cell cycle arrest. A, Down-regulation of ERα by 10 nM ICI182780 treatment for 60–96 hrs. ERα and Ki-67 were evaluated by immunofluorescent staining, nuclei were stained with DAPI. Cells without ICI182780 treatment were used as control. Magnification, 200×. B, flow cytometry analysis of cell cycle distribution. Shown here is the cell cycle distribution of non-treated control cells, and cells treated with 10 nM ICI182780 for 48–72 hr. ICI182780 treatment was carried out in phenol-red free DMEM/F12 containing 5% CD-FBS.