Figure 2

Deregulated expression of ERα in the cell cycle in MCF-7 cells. A, co-immunofluorescent staining for ERα (green fluorescence) and the major cyclins (D, E, A, and B) (red fluorescence). Cells were immunofluorescent stained with antibody for ERα and the antibody for one of the cyclins, followed by secondary antibody. Colocalization was shown by overlaying (Merge). Magnification, 200×. B, Flow cytometry analysis for the cell cycle distribution of ERα^high cells (cells with high levels of ERα). Cells were stained with antibody for ERα followed by Alexa Fluor 488, and nuclear DNA was stained with propidium iodide (PI).