Heparanase expression and activity in rhabdomyosarcoma patients. (A) HPSE mRNA expression levels in foetal skeletal muscle (white bar) and RMS patients (black bar) were determined by Real-time PCR. The results were normalized using GAPDH as internal control and represent the mean ± standard deviation of two independent experiments performed in triplicate. (B) HPSE activity in each plasma sample was calculated as difference between the O.D. 405 value with or without heparin at the final concentration of 50 μg/ml. An asterisk (*) indicates a significant difference between RMS patients and controls (P < 0.05).