Matrix Metalloproteinases expression in RH30 and RD cell lines. MMPs mRNA expression levels in control (black and gray bars) and silenced (hatched bars) RH30 and RD cell lines were determined by Real-time PCR. The results were normalized using GAPDH as internal control. The expression level determined for MMP2 in RH30 wild type cells is regarded as 100% and MMPs expression in the other cell lines are presented as percentage relative to it. The results represent the mean ± standard deviation of two independent experiments performed in triplicate.