Figure 5

Migration of glioma cells following MGP knockdown using ChemoTX migration chambers. (a) Quantification of cell migration: U373fast, H4 and U343MG cells with reduced MGP expression exhibited reduced migration as compared to control cells transfected with non-silencing siRNA (set at 100%) 24 h after seeding in the upper chamber (all differences between siRNA and control transfected cells are significant with p < 0.001 = ***). b-d: Representative migration chamber assay using H4 cells. Reduced numbers of H4 cells were observed on the lower side of the filter following knockdown with MGP specific siRNA (c = siRNA MGP_4, d = siRNA MGP_8) as compared cells transfected with non-silencing siRNA (b). Nuclei were counterstained with Hoechst 33258.