Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: Matrix gla protein (MGP): an overexpressed and migration-promoting mesenchymal component in glioblastoma

Figure 3

MGP knockdown using siRNA. (a) Transfection of U373fast, H4 and U343MG cells with two different siRNAs targeting MGP mRNA resulted in decreased MGP expression at mRNA levels at different time points following transfection, as determined by quantitative RT-PCR. MGP mRNA is expressed relative to control transfections using non-silencing siRNA (set at 100%). b-d: Knockdown verification on protein levels by immunofluorescence staining revealed decreased fluorescence intensities in U373fast cells for both siRNAs 72 h post-transfection (c, d) compared to control transfected cells (b) (MGP is marked green in the cytoplasm). Similar results were obtained for H4 and U343MG cells. Nuclei were counterstained with Hoechst 33258 (blue).

Back to article page