Figure 2

Cellular localization of MGP protein. Immunohistochemistry revealed positivity of tumor cells (a, b, arrow), while neurons of the normal neocortex were faintly positive (c, arrow) and glial cells were negative. An atheromatous plaque, used as positive control, showed distinct staining in the media (d, arrow). e-g: Immunofluorescence of glioblastoma cell lines U373fast (e), H4 (f) and U343MG (g) showed cytoplasmic positivity. Negative controls using nonspecific IgG remained unlabeled (h). Bar = 100 μm (a-d) and 50 μm (e-h).