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Figure 1 | BMC Cancer

Figure 1

From: Activation of the steroid and xenobiotic receptor, SXR, induces apoptosis in breast cancer cells

Figure 1

SXR mRNA and protein are expressed in breast cancer cell lines. A) Total RNAs from breast cancer cell lines, MCF-7 and ZR-75-1 and colon carcinoma cell line LS180 were isolated, reverse transcribed and subjected to QRT-PCR analysis using primers for human SXR. Values represent the average of triplicates ± S.E.M. and were replicated in at least three independent experiments. B) Equal amounts of total cell lysates (30 μg-in each lane) were separated by SDS-PAGE followed by Western blot analysis using the rabbit polyclonal anti-SXR (anti-412) antibody. The blot was stripped and re-probed with anti-GAPDH antibody (mouse monoclonal 6C5, Ambion, Inc., Austin, TX) as a loading control. The results were replicated in at least three independent runs.

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