Figure 1From: Activation of the steroid and xenobiotic receptor, SXR, induces apoptosis in breast cancer cells SXR mRNA and protein are expressed in breast cancer cell lines. A) Total RNAs from breast cancer cell lines, MCF-7 and ZR-75-1 and colon carcinoma cell line LS180 were isolated, reverse transcribed and subjected to QRT-PCR analysis using primers for human SXR. Values represent the average of triplicates ± S.E.M. and were replicated in at least three independent experiments. B) Equal amounts of total cell lysates (30 μg-in each lane) were separated by SDS-PAGE followed by Western blot analysis using the rabbit polyclonal anti-SXR (anti-412) antibody. The blot was stripped and re-probed with anti-GAPDH antibody (mouse monoclonal 6C5, Ambion, Inc., Austin, TX) as a loading control. The results were replicated in at least three independent runs.Back to article page