Figure 4

CD25 expression and viability of lymphocytes after co-culture. (A and B) Lymphocytes co-cultured with macrophages for 24 h (treated or untreated) without cell contact (0.4 μm insert). In Ly/Mg* was observed a higher amount of unviable (7-AAD⁺) Ly cells in control group (Fig. A). Unviable cells were not included in CD25 analysis for to keep comparison between viable CD25+ cells; (C and D) melanoma cells co-cultured with lymphocytes previously co-cultured with macrophages. Percentage of CD25 and viability (7-AAD^low) subpopulations in lymphocytic cells are shown at the quadrant extremities.