Figure 2
From: Vasohibin inhibits angiogenic sprouting in vitro and supports vascular maturation processes in vivo
Effects of recombinant vasohibins on angiogenic sprouting of endothelial cells. (A) HUVEC spheroids were stimulated for 24 h with VEGF and bFGF in the presence of control FLAG protein to induce angiogenic sprouting into the collagen gel. (B) Angiogenic sprouting was inhibited by administering of 10 nM recombinant murine VASH1. (C) Effects of recombinant human and murine VASH1 (10 nM) on bFGF-induced angiogenic sprouting. (D) Effects of recombinant human and murine VASH1 (10 nM) on VEGF-induced angiogenic sprouting. FLAG indicates the control extract of the affinity purification. CSL indicates the cumulative sprout length of the spheroid and stars indicate p values < 0.05.