Figure 1
From: Vasohibin inhibits angiogenic sprouting in vitro and supports vascular maturation processes in vivo
Effects of recombinant vasohibins on cell viability of fibroblasts, smooth muscle and endothelial cells. (A) Affinity purification of FLAG-tagged vasohibin proteins. Cell extracts of B16F10 cells with stable expression of human and murine VASH1 in comparison to wild-type cells (WT). Recombinant human and murine VASH1 proteins were analyzed by Western Blot after purification from cell extracts by affinity chromatography. Unspecific proteins of WT cells binding to the column are indicated as control. (B) Embryonic mouse fibroblasts (mFB), mouse aortic smooth muscle cells (mASMC), human umbilical vein (HUVEC) and microvascular endothelial cells (HMEC) were stimulated with 10 nM murine or human VASH1 protein or control extract (FLAG), after which apoptotic cells were counted by flow cytometry after Annexin-V/7AAD staining. Untreated cells were used as control. (C) Cell migration was analyzed in the scratch assay 6 h after stimulation with 10 nM mouse or human VASH1 or control protein. Stars indicate p values < 0.05.