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Figure 5 | BMC Cancer

Figure 5

From: The cyclin-dependent kinase inhibitor 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole induces nongenotoxic, DNA replication-independent apoptosis of normal and leukemic cells, regardless of their p53 status

Figure 5

In the absence of p53, DRB-induced apoptosis is mediated by p73. (A) Analysis of p53 on Jurkat cells. Western blot analysis was performed on control and Jurkat T cells harvested before or 3 hr after 2 Gy γ-IR. (B) DRB induces Jurkat cell death. Jurkat cells were kept in medium alone or containing 10–100 μM DRB, harvested at 24, 48 and 72 hr and analyzed by flow cytometry after staining with PI. p < 0.05 with the test of independence at doses above 40 μM, compared to untreated cells. (C) DRB induces Jurkat apoptosis. Jurkat cells were harvested before or 24 hr after 10–100 μM DRB and stained with anti-active caspase-3 antibody (left panel) and with annexin V and PI (right panel). Histograms show the percentage of active caspase-3+ cells (mean ± S.D.) and PI- annexin V+ cells. (D) Immunoblot analysis of p73 and p63 on Jurkat cells harvested before (0) or after 100 μM DRB. p73 and p63 positive controls (+ lane): whole cell lysate from HL60 and TAp63α transfected 293T cells, respectively. (E) Inhibition of p53 in the p53 wild-type tumour cell line MOLT-4 induces p73 activation. Western blot analysis of MOLT-4 cells harvested before (0) or after 100 μM DRB alone (left panel) or following preincubation with α-PFT and μ-PFT (right panel).

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