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Figure 2 | BMC Cancer

Figure 2

From: The cyclin-dependent kinase inhibitor 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole induces nongenotoxic, DNA replication-independent apoptosis of normal and leukemic cells, regardless of their p53 status

Figure 2

DRB-induced apoptosis is replication-independent, DNA damage-independent and p53-mediated. (A) DRB treatment does not induce entry into S phase. Circulating lymphocytes from one healthy donor were maintained in medium alone or containing 100 μM DRB, harvested 3, 6, 9, 24 and 48 hr after treatment, fixed and analysed for cell cycle. Histograms show the percentage of cells in subG0/G1 (white bars), in G0/G1 (black bars) or in S-G2/M (grey bars) phases of the cell cycle at each treatment time. (B) DRB treatment does not induce DSBs. Cultured T cells from one donor were maintained in medium alone, supplemented with the caspase inhibitor ZVAD or supplemented with 100 μM DRB and ZVAD. Part of the cells kept in medium alone were irradiated with 2 Gy γ-IR. Cells were harvested 24 hr after 100 μM DRB or 1 hr after 2 Gy γ-IR and stained for γ-H2AX. One representative experiment out of two is shown. (C) Immunoblot analysis of p53, p53pSer15 and Mdm2 following DRB. Time course analysis of normal T cells harvested before (0) or at various times after 100 μM DRB treatment. One representative experiment out of three is shown.

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