Figure 2

AP-2γ binds to genomic DNA fragments near to ErbB2, CDH2, HPSE and IGSF11 genes in vivo. Normal rabbit IgG and rabbit polyclonal anti-AP-2γ antibody were used to immunoprecipitate protein-bound DNA in MDA-MB-453 cells. The immunoprecipitated genomic DNA was subjected to PCR amplification using primers flanking the AP-2γ response elements. Genomic DNA (Input) without immunoprecipitation was used as positive controls, and housekeeping gene β-actin was used as negative control.