|
Sample†
|
Preparation* Protocol
|
ER
|
PR
|
HER 2/nu
|
Pathologic diagnosis
|
|---|
| | |
Normal
|
Cancer
|
Normal
|
Cancer
|
Normal
|
Cancer
| |
|---|
|
1A
|
1
|
NA
|
+
|
NA
|
+
|
NA
|
-
|
DCIS
|
|
1B
|
2
|
NA
|
+
|
NA
|
-
|
NA
|
-
|
DCIS
|
|
1C
|
3
|
NA
|
+
|
NA
|
+
|
NA
|
-
|
DCIS
|
|
2A
|
1
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
2B
|
4
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
2C
|
5
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
3A
|
1
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
3B
|
6
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
3C
|
5
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
5A
|
1
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
5B
|
5
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
5C
|
4
|
NA
|
-
|
NA
|
-
|
NA
|
+
|
DCIS
|
|
6A
|
1
|
+
|
-
|
+
|
-
|
NA
|
-
|
Invasive DC
|
|
6B
|
4
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
6C
|
5
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
6D
|
8
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
7A
|
1
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
7B
|
9
|
-
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
7C
|
10
|
-
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
7D
|
5
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
8A
|
5
|
+ weak
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal (atrophic)
|
|
8B
|
1
|
+ weak
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal (atrophic)
|
|
8C
|
1
|
+ weak
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal (atrophic)
|
|
9A
|
5
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
9B
|
1
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
9C
|
1
|
NA
|
-
|
NA
|
-
|
NA
|
-
|
Invasive DC
|
|
10A
|
5
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
10B
|
3
|
+
|
NA
|
+
|
NA
|
NA
|
NA
|
Normal
|
|
10C
|
11
|
+
|
-
|
+
|
-
|
NA
|
-
|
Normal and DCIS
|
|
10D
|
1
|
+
|
-
|
+
|
-
|
NA
|
-
|
Normal and DCIS
|
- †While 10 cases were studied, sample 4 was excluded due to inadequate tissue handling. * Preparation Protocol: Preparation 1: Control; Preparation 2: Unbuffered 10% citric acid (1 min) and saline bath (5 min); Preparation 3: Buffered 10% citric acid (1 min) and glycerin (3 min) without saline; Preparation 4: Glycerin (3 min) without saline; Preparation 5: Buffered 10% citric acid (1 min) and glycerin (3 min) and saline bath; Preparation 6: Buffered 10% citric acid (1 min) without saline bath; Preparation 7: 5% Acetic acid (5 min) without saline bath; Preparation 8: Glycerin (3 min) with saline bath (5 min); Preparation 9: Unbuffered 10% citric acid (1 min) and glycerin (3 min) with saline bath (5 min); Preparation 10: 5% Acetic acid (1 min) and glycerin (3 min) without saline bath; Preparation 11: Mixture of buffered 10% citric acid and 5% acetic acid (1 min) and glycerin (3 min) without saline bath.
