Skip to main content
Figure 6 | BMC Cancer

Figure 6

From: Citrus auraptene suppresses cyclin D1 and significantly delays N-methyl nitrosourea induced mammary carcinogenesis in female Sprague-Dawley rats

Figure 6

Concentrations of auraptene in rat tissues from the tumor study. The rat mammary glands, tumors and livers were weighed and homogenized. To the homogenate 80 μL of 100 μM curcumin was added as internal standard and extracted twice with ethyl acetate: acetone mixture (2:1 v/v). The extract was dried and resuspended in 1 mL 70% methanol/H2O and analyzed by HPLC (Shimadzu, SIL-10AF) with UV (254 nm) and fluorescence detection (Shimadzu, RF-535) at excitation and emission wavelengths of 329 nm and 390 nm respectively.

Back to article page