Figure 2

Effects of auraptene on human breast carcinoma cells. (A) Effects of auraptene on MDA-MB-231 cell proliferation. Cells were serum starved for 5 h and then treated with 1–50 μM auraptene in 0.01% DMSO. Cell proliferation was assessed using the MTT reagent. Figures represent % vehicle control (means ± S.E.) # Replicates = 2 plates at 6 wells each for the treatment groups and 12 wells for the control group (* statistically different from control group, p < 0.05, One-way ANOVA, Tukey test). (B) Effects of auraptene on MCF-7 cell proliferation. Cells were serum starved for 24 h. At 22 h after serum starvation the cells were treated with 1–50 μM auraptene in 0.01% DMSO. At 24 h serum starvation, the cells were treated with IGF-1 (10 ng/mL). Cell proliferation was assessed using the MTS reagent. Figures represent % vehicle control (mean± S.E.) # Replicates = 2 plates at 6 wells each for the treatment groups. (* statistically different from IGF-1 group, p < 0.05, One-way ANOVA, Tukey test). (C) Effects of auraptene on IGF-1 induced cyclin D1 expression. MCF-7 cells were serum starved for 24 h. At 22 h after serum starvation the cells were treated with10 μM auraptene in 0.01% DMSO. At 24 h serum starvation the cells were treated with IGF-1 (10 ng/mL). The cells were harvested at the 15, 30 minutes and at 1 h, 2 h, 4 h, 8 h and 24 h. The cell lysates were probed for cyclin D1. The figure shows a representative blot. The lower band (36 KD) is cyclin D1 and the upper band is β-actin (43 KD).