Figure 3
From: Role of KCNMA1gene in breast cancer invasion and metastasis to brain
Localization and expression of BK Ca channel protein. A: Immunofluorescence of breast cancer cell lines stained for BKCa channel (green) and nuclei with DAPI (blue). Expression of BKCa channel was strong in MDA-MB-231 and MDA-MB-361, weak in MCF-7 and indiscernible in MCF-10A. Stains were repeated twice. B: Flow cytometric detection of BKCa channel protein expression in various breast cell lines. Representative histograms from (a) MCF-10A, (b) MCF-7, (c) MDA-MB-231, and (d) MDA-MB-361 showing expression of BKCa channel protein. C: Immunofluorescence of anti-BKCa channels antibody on human breast cancer tissue. Antibody labeled in green (FITC), nuclei counterstained in blue (DAPI). (a) Very few cells show BKCa channels staining in the primary breast cancer tissue (n = 6, See large arrow). Note: Absence of green staining in most of the cells also indicates specificity of the antibody (See small arrows). (b) Systemic metastatic breast cancer (lymph node) shows an increased number of cells stained with BKCa channel antibody (n = 6; See large arrows). (c) All breast cancer tissue metastatic to the brain are strongly positive for the BKCa channels (n = 4). Magnification = 400×. D: Representative H&E staining of breast tumor tissue sections.