Figure 3
From: Claudin-containing exosomes in the peripheral circulation of women with ovarian cancer
The kinetics of claudin release. A, BG-1 cells were pulsed-labeled with [35S]methionine and [35S]cysteine, chased for various times as indicated, before the media was collected. Claudin-4 was immunoprecipited from the media or from the [35S]-labeled whole cell lysate (as control for the total amount of labeled claudin-4) and analyzed by SDS-PAGE. Claudin-4 is released rapidly in the media, with radiolabeled claudin-4 detectable as early as 2 hours post-labeling. B, The radiolabeled claudin-4 released into the culture media was quantitated by phosphorimaging of the blot shown in (A) and plotted against time. Claudin-4 increase in the media is linear with time.