Figure 1
From: Claudin-containing exosomes in the peripheral circulation of women with ovarian cancer
Claudin proteins are shed from cancer cell lines. A, Cell lysates and culture media were collected from ovarian cancer cell lines, and analyzed by immunoblotting for the expression of claudin-1, claudin-3, claudin-4 and claudin-5. Ovarian cancer cell lines used: OVCAR-2 (1), OVCAR-3 (2), OVCA420 (3), OVCA433 (4), BG-1 (5), Hey (6), UCI101 (7), and A2780 (8). B, Cell lysates and culture media were collected from various cancer cell lines, and analyzed by immunoblotting for the expression of claudin-1, claudin-3, claudin-4 and claudin-5. Cell lines analyzed are: colon cancer HCT-116 (1), colon cancer SW-480 (2), breast cancer MCF-7 (3), and ovarian cancer OVCAR-3 (4). Claudins expressed in the various cell lines were released in the media.