A novel chalcone derivative which acts as a microtubule depolymerising agent and an inhibitor of P-gp and BCRP in in-vitro and in-vivoglioblastoma models

Table 1 Percentage of cells in the cell cycle phases after 24 hours incubation with 10 μM JAI-51

Cell cycle phase	U118C	U118T	U138C	U138T	U373C	U373T	LN229C	LN229T	GL26C	GL26T
G0/G1	60 ± 9	30 ± 5 *	54 ± 6	32 ± 6 *	54 ± 4	21 ± 3 *	53 ± 5	12 ± 4 *	55 ± 8	30 ± 5 *
S	18 ± 2	15 ± 3	10 ± 2	9 ± 2	20 ± 2	15 ± 2	18 ± 3	19 ± 4	22 ± 3	10 ± 3
G2/M	22 ± 3	55 ± 7 *	34 ± 7	58 ± 6 *	26 ± 3	64 ± 6 *	28 ± 3	68 ± 6 *	23 ± 4	60 ± 8 *

Glioblastoma cells were cultured for 24 hours without (C) or with 10 μM JAI-51 (T) before being stained with propidium iodide. DNA content was analyzed by flow-cytometry and the percentage of cells in each phase of the cell cycle was obtained by using Modfit program. Numbers ± SD indicate the percentage of cells and are the mean of 3 to 5 experiments. * P < 0.05 (treated versus control).

ISSN: 1471-2407