Focal adhesion staining of twist expressed cells. CAT (control) and twist cDNAs were introduced in PLC, HepG2, and Huh7 cells using Superfect™ chemical transfection reagent. After a 48-h incubation period, cells were fixed. Focal adhesion complexes were visualized by immunofluorescence staining of vinculin (green), as described in Materials and Method. Actin stress fibers were detected with rhodamine-phaloidin (red). A. Representative fields from three independent experiments in PLC are shown. B. Focal adhesion complexes in each cell were counted. All results are expressed as a ratio to control (CAT) cDNA transfected PLC (CAT; clear bars, twist; black bars). The data are the mean ± SEM of at least three independent studies each performed in triplicate. Statistical analysis was performed by Student's t-test: **P < 0.01 (vs CAT cDNA transfected cells).