Figure 3

MSP assessment of tumors versus normal tissues of three patients along with controls. Lanes 1, 3 and 5 show unmethylated APC promoter amplification product of normal nonmalignant tissues with a 111 bp PCR product. Lanes 2, 4 and 6 are the corresponding methylated tumor tissues of APC promoter as could be observed with 98 bp PCR product. Lane 7; negative control (blood) which results in 111 bp product. Lane 10; positive control (universally methylated DNA) with a 98 bp product. Lane M; molecular size marker 50 bp (Fermentase).