Figure 3

MYCN , ΔMYCN , and MYCNOS mRNA expression levels in NBs. (A) Expression levels of MYCN, ΔMYCN and MYCNOS in MYCN-amplified (closed-triangles) compared to non-amplified (open triangles) tumours. (B) Relative expression-levels of MYCN (closed triangles), ΔMYCN (closed circles) and MYCNOS (open circles) correlated to MYCN-amplification in NBs. (C) Difference of the MYCN:MYCNOS-ratio between NBs with MYCN-amplification (closed circles) and NBs without MYCN-amplification (open circles). (D) Correlation of the number of MYCN copies with MYCN:MYCNOS mRNA ratios (closed circles and non-interrupted line) and with MYCN:MYCN mRNA ratios (open triangles and dotted line). (E) Correlation between NB stage and MYCN:MYCNOS-ratio. (F) Correlation between NB stage and MYCN: ΔMYCN-ratio. NB IV-s is a special type of NB characterized by metastatic disease with spontaneous regression and good survival [31].