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Figure 2 | BMC Cancer

Figure 2

From: Regulation of MYCNexpression in human neuroblastoma cells

Figure 2

Detection of MYCN and ΔMYCN in IMR-32 cells. (A) Western blot, visualizing two proteins in the IMR-32 (NB cell line with MYCN-amplification) lysate with the C-19 antibody that recognizes the C-terminal epitope of both MYCN and ΔMYCN. In the BLM (melanoma cell line without MYCN-amplification) lysate, these proteins were not present. (B) Western blot of IMR-32 whole lysate (1), whole lysate minus precipitate (2) and the precipitate (3) using the C-19 antibody. Arrows indicate the positions of MYCN (65 kDa) and ΔMYCN (45 kDa). The additional band in lane 3 is caused by deposition of Ig-heavy chains (50 kDa). Exposure times are indicated below the blots.

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