Table 1 Antibodies and probes used for fluorescent localization.
Primary antibodies and probes | |||
|---|---|---|---|
Antibody/Probe | Species | Source | Dilution |
E-cadherin (HECD1) | Mouse, hybridoma supernatant | Dr. Alpha Yap (Inst. Mol. Sci., University of QLD) | 1:2 |
Vimentin (V-9) | Mouse | Sigma-Aldrich | 1:400 |
Paxillin (349) | Mouse | BD Biosciences | 1:500 |
Phalloidin (F-actin) | Fungus | Molecular Probes (Invitrogen) | 1:400 |
Secondary antibodies | |||
Anti-mouse: Alexa 488 | Goat | Molecular Probes (Invitrogen) | 1:2000 |