Figure 1
From: Identification of Makorin 1 as a novel SEREX antigen of esophageal squamous cell carcinoma
Recognition of MKRN1 by serum antibodies in patients with esophageal SCC. Escherichia coli containing MKRN1 cDNA expression plasmid (MKRN1) or control empty plasmid (Control) was treated with (+) or without (-) IPTG for 2.5 h, and cell lysates were subsequently subjected to Western blot analysis using the sera from healthy donor #1 and esophageal SCC patients #15 as indicated. An arrow indicates the IPTG-induced polypeptide that represents the encoded MKRN1 cDNA product.