Differential regulation of key markers of VHL function by type 1 and type 2 VHL mutants. (A) Indicated RCC10 cell lines were grown to confluence on collagen I (under standard serum conditions) and prepared cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for α5 and β1 integrins, cyclin D1, p27, and α-tubulin. VHL subtypes that mutations represent are provided at the top. Quantification of the band intensities for p27 blot (as a percent of the band with greatest intensity) is provided at the bottom. (B) A498 cells lines were similarly grown and analyzed by western blotting. VHL subtypes that mutations represent are provided in parentheses.