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Table 1 Primer sequences for RT-PCR

From: Downregulation of SFRP5 expression and its inverse correlation with those of MMP-7 and MT1-MMP in gastric cancer

Gene   Sequence Product size Annealling temperature
MMP-2 Sense 5'-GGATGATGCCTTTGCTCG-3' 487 bp 55°C
  Anti-sense 5'-ATAGGATGTGCCCTGGAA-3'   
MMP-7 Sense 5'-CTTCAGGCAGAACATCCA-3' 220 bp 49°C
  Anti-sense 5'-ATTTATTGACATCTACCCAC-3'   
MMP-9 Sense 5'-AGGACGGCAATGCTGATC-3' 127 bp 58°C
  Anti-sense 5'-TCGTAGTTGGCGGTGGTG-3'   
MT1-MMP Sense 5'-CCTGCGTCCATCAACACT-3' 481 bp 57°C
  Anti-sense 5'-TCACCTCCGTCTCCTCCTC-3'   
SFRP5 Sense 5'-AGGGTAAGGGAAAGGTGGAG-3' 379 bp 60°C
  Anti-sense 5'-GAGAAGCAGAGGCTGAGGAA-3'   
GAPDH Sense 5'-GGGAAACTGTGGCGTGAT-3' 309 bp 56°C
  Anti-sense 5'-AAAGGTGGAGGAGTGGGT-3'