Figure 6

Transfecting katoIII with pcDNA3.1 SFRP5 vector. (A) Increased expression of SFRP5 mRNA was detected in katoIII after SFRP5 transfection treatment by RT-PCR. (B) Increased expression of SFRP5 protein was detected after SFRP5 transfection treatment by Western blotting. (C) Real-time PCR analysis showed MMP-7 mRNA and MT1-MMP mRNA were downregulated in katoIII after SFRP5 transfection treatment. Data are expressed as mean ± SD, n = 3, *P < 0.001, vs Empty vector. (D) Weatern blotting analysis showed MMP-7 protein and MT1-MMP protein were downregulated in katoIII after SFRP5 transfection treatment.