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Figure 2 | BMC Cancer

Figure 2

From: Downregulation of SFRP5 expression and its inverse correlation with those of MMP-7 and MT1-MMP in gastric cancer

Figure 2

SFRP5 expression and SFRP5 methylation in gastric cancer cell lines. (A) SFRP5 mRNA was not detected in MKN28, MKN45, AGS, NCI-N87 and HGC-27 by RT-PCR. (B) SFRP5 protein was not detected in MKN28, MKN45, AGS, NCI-N87 and HGC-27 by Western blotting. (C) SFRP5 methylation was found in MKN28, MKN45, AGS, NCI-N87 and HGC-27 by methylation-specific PCR. (D) SFRP5 mRNA re-expression was found in MKN28, MKN45, AGS, NCI-N87 and HGC-27 after treatment with DAC by RT-PCR. (E) SFRP5 protein re-expression was found in MKN28, MKN45, AGS, NCI-N87 and HGC-27 after treatment with DAC by Western blotting. M: methylated primers; U: unmethylated primers.

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