Sequence (5' → 3') | TA [°C] | Primer [nM] | Product size (bp) | |
---|---|---|---|---|
Methylation-specific PCR | ||||
WIF1 unmethylated | Forward: GGGTGTTTTATTGGGTGTATTGT | 55 | 400 | 154 |
Reverse: AAAAAAACTAACACAAACAAAATACAAAC | ||||
WIF1 methylated | Forward: CGTTTTATTGGGCGTATCGT | 55 | 400 | 145 |
Reverse: ACTAACGCGAACGAAATACGA | ||||
DKK3 unmethylated | Forward: TTAGGGGTGGGTGGTGGGGT | 58 | 320 | 126 |
Reverse: CTACATCTCCACTCTACACCCA | ||||
DKK3 methylated | Forward: GGGCGGGCGGCGGGGC | 58 | 320 | 120 |
Reverse: ACATCTCCGCTCTACGCCCG |