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Table 1 Oligonucleotide primers used in the study

From: Prognostic relevance of Wnt-inhibitory factor-1 (WIF1) and Dickkopf-3 (DKK3) promoter methylation in human breast cancer

 

Sequence (5' → 3')

TA

[°C]

Primer

[nM]

Product size (bp)

Methylation-specific PCR

WIF1 unmethylated

Forward: GGGTGTTTTATTGGGTGTATTGT

55

400

154

 

Reverse: AAAAAAACTAACACAAACAAAATACAAAC

   

WIF1 methylated

Forward: CGTTTTATTGGGCGTATCGT

55

400

145

 

Reverse: ACTAACGCGAACGAAATACGA

   

DKK3 unmethylated

Forward: TTAGGGGTGGGTGGTGGGGT

58

320

126

 

Reverse: CTACATCTCCACTCTACACCCA

   

DKK3 methylated

Forward: GGGCGGGCGGCGGGGC

58

320

120

 

Reverse: ACATCTCCGCTCTACGCCCG

   
  1. TA, annealing temperature.