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Figure 1 | BMC Cancer

Figure 1

From: Prognostic relevance of Wnt-inhibitory factor-1 (WIF1) and Dickkopf-3 (DKK3) promoter methylation in human breast cancer

Figure 1

Methylation analysis of the human WIF1 promoter. (A) A 1.29 kb genomic sequence of the WIF1 promoter, analyzed by methprimer software [29], revealed the presence of a CpG island (blue) between relative position 604 and 1153. Position 1000 indicates the transcription start site (TSS, arrow). A region of high CpG (red vertical bars) densitiy was chosen for MSP analysis. The black bar indicates the MSP amplicon. (B) Sensitivity of the utilized MSP primers was determined by a dilution series of methylated DNA with unmethylated DNA (Epi Tect control DNA, Qiagen). At least 1% of methylated DNA (~0.1 ng) can be detected with the WIF1 MSP primers. bp, base pair marker; NTC, 'no template control'.

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