COX-2 dependent generation of PGE
and signalling through the EP4 receptor. (2a) Generation of PGE2 in HT-29 cells over 15 minutes following treatment with various doses of the COX-2 inhibitor SC-236 or Aspirin (ASA, 200 μM) for 4 hours. Values are the mean +/- standard error of the mean (n = 3). One way ANOVA p = 0.0001; * p = 0.01 ** p = 0.001 (Bonferroni multiple comparisons test between selected groups). (2b) Intracellular cAMP concentrations following treatment with SC236 (5 μM) or L-161–982 (10 μM) for 30 mins. The chart shows mean values +/- SEM for n = 3. One way ANOVA (overall) p = 0.01, p values shown are for Bonferroni multiple comparisons test between selected groups.