A. RFLPs of PCR-amplified fragments obtained with HaeIII. There are 2 genotypes represented by the gel picture, wild type (a, c and d) where a 158 bp band is absent and 249Ser (b, e and f) where the 158 pb fragment can be observed. 1B. Representative eletropherograms of TP53 sequence. Nucleotide sequence of a mutated sample on top, showing the G → T transversion (AGT) when compared with a wild type exon 7 below (AGG).