The levels of prostasin mRNA were determined by real-time RT-PCR from healthy individuals (N), individuals with mild/moderate dysplasia (M/M), severe dysplasia (S) or carcinomas (C). Both normal (grey bars) and affected (white bars) tissues were examined from each individual. From colorectal cancer patients one sample of histological normal tissue was taken as far away from the colorectal cancer tissue as allowed by the surgically removed tissue (normal distant, here designated ND) and one sample of histological normal tissue was taken immediately adjacent to the colorectal cancer tissue (normal adjacent here designated NA). mRNA levels were normalised to the β-actin mRNA level. The box shows the interquartile range, the whiskers the range and the median is indicated by a vertical line.