Figure 3

cDNA dot blot analysis of RAI3 expression in matched normal and t breast tissue. Expression profiles were determined using cDNA dot blot hybridisation analysis (BD Clontech) containing cDNA pairs derived from 50 matched normal breast tissues (N), tumourous breast tissues (T), and three lymph node metastastic tissues (marked with arrows). Upregulation of RAI3 was observed in 30 of 50 primary breast tumours as well as in one of three metastatic lymph nodes, as compared to matched normal breast tissue.